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Melior Pharmaceuticals

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery cardiovascular

Bleeding Time / Anti-Thrombotic Assay
Thrombosis is defined as the formation or presence of a blood clot inside a blood vessel. This clotting can be deleterious and evoke events such as stroke and heart attacks. Anti-coagulants and anti-thrombotic agents are used to prevent this unwanted blood clotting and are commonly used prophylactically in prothrombotic situations. Bleeding time assays in rodents can be used to predict the anti-thrombotic/anti-coagulant potential of test agents. In this assay, we established a common bleeding time model and used Heparin to validate and benchmark this model.

                           Bleed time anti-thrombosis heparin blood clot anti-coagulant

Latency to Clot. CD1 mice were treated with vehicle or Heparin five minutes prior to testing.  The animals' tails were then cut and placed in a flask containing 37°C water.  Latency to clot was measured in seconds with a 600 second time out.  The Heparin group displayed an increased latency to clotting time compared to vehicle.  Data are mean ± SEM analyzed using an unpaired t-test; **p<0.01 compared to vehicle.

 

If you are interested in learning more about the Bleeding Time/ Anti-Thrombotic Assay, please contact models@meliordiscovery.com to start the conversation.