melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling
Melior Discovery
home
Therapeutic Areas
Safety Assessment
 

 

theraTRACE

Melior Pharmaceuticals

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery gastrointestinal

Colonic Propulsion

This gastrointestinal transit model is a method for evaluating a test compounds' ability to alter colonic propulsive motility.  This model is designed to identify pharmacological agents that produce inhibitory effects on propulsive motor activity and is an excellent predictor of drugs that may be useful for the therapy of irritable bowel syndrome (IBS).  This model can be modified to identify antagonists of constipative drugs or diarrhea producing drugs.  In this validation study, colonic propulsion was measured in mice treated with morphine, a well-described compound that reduces colonic propulsive activity.

 

                           Colonic propulsion colonic motility morphine

 

Latency to bead expulsion.  CD1 mice (n=10) were injected with either vehicle or Morphine subcutaneously and thirty minutes later a 3mm glass bead is inserted into the distal colon of a mouse.  Mice were monitored for up to thirty minutes and latency to bead expulsion was recorded.  Morphine treated mice displayed a significantly higher latency to bead expulsion compared to vehicle treated animals.  Data are mean ± SEM and evaluated using unpaired t-test; **p<0.01 compared to vehicle.

 

Morphine treated animals can be used to look for antagonists of morphine-induced constipative effects which, in humans, is problematic in extending hospital stays.  This study validates the colonic expulsion assay and demonstrates that Morphine, a well-described inhibitor of colonic propulsion, significantly attenuated propulsive activity.

 

If you are interested in learning more about the Colonic Propulsion Assay, please contact models@meliordiscovery.com to start the conversation.