melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling
Melior Discovery
home
Therapeutic Areas
Safety Assessment
 

 

theraTRACE

 

theraTRACE

Melior Pharmaceuticals

              

 

             

 

                 

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery gastrointestinal

Morphine - Induced Constipation
This gastrointestinal transit model is a method for evaluating a test compound's ability to alter colonic propulsive motility.  This model is designed to identify pharmacological agents that can functionally antagonize the constipative activities produced by morphine as measured by an increase in colonic propulsive activity.  This model can be used to identify drugs that directly affect morphine receptors or that functionally affect morphine-induced colonic activity.  In this study, we tested the ability of Naloxone, a well-known mu-opioid receptor antagonist, on morphine-induced reductions in colonic propulsive activity. 

                     Morphine induced constipation naloxone treatment

Mice were fasted 24 hours prior to study commencement.  Mice were treated with either vehicle or Naloxone 30 minutes prior to Morphine challenge.  Mice were then administered Morphine 30 minutes prior to the colonic propulsion study.  At peak drug time, a 3 mm glass bead was inserted through the anus into the distal colon.  Mice were observed for the expulsion of the bead and the time noted, with a cutoff of 30 minutes. 

 

Naloxone, a well-known mu-opiod receptor antagonist, alleviated the time to colonic propulsion and constipation. Mice treated with vehicle had a significantly increased expulsion time compared to Naloxone-treated mice. Data are mean ± SEM; **p<0.01 compared to control mice.

 

If you are interested in learning more the Morphine-Induced Constipation assay, please contact models@meliordiscovery.com to start the conversation today.