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Melior Pharmaceuticals

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery inflammation/arthritis

Delayed Type Hypersensitivity
Delayed type hypersensitivity (DTH) reactions are antigen-specific cell-mediated immune responses that can invoke harmful (e.g., allergic dermatitis and autoimmunity) aspects of immune function. The immune reaction induced by the method described below is characterized by swelling at the site of challenge and by an infiltration of monocytes/macrophages and lymphocytes into the epidermis and dermis. This model for skin DTH reactions has been used widely to monitor cell-mediated immune responses in vivo.   Melior has establishe a model of delayed type hypersensitivity using sheep red blood cells (SRBC) as the antigen.  Dexamethasone, a steroid anti-inflammatory, was employed to pharmacologically validate the assay.

                         delayed type hypersensitivity

On Day 1 of the study, mice were IV injected with washed sheep red blood cells (SRBC).  On Day 6 of the study, mice were treated with either vehicle or Dexamethasone followed by  injection of sheep red blood cells (SRBC) into the paws of sensitized mice producing an allergic reaction as measured by a digital caliper.  Footpad thickness per animal was normalized to the uninjected (back right) footpad. .

 

Injection of sheep red blood cells into the paws of sensitized mice produced an allergic reaction as measured by swelling (paw thickness).  Dexamethasone, a steroid anti-inflammatory, significantly reduced foot pad swelling as measured using a microcaliper. Data are mean ± SEM; ***p<0.001 compared to vehicle.

 

The mouse DTH model is a test against an immunological inflammation response.  Animals that received vehicle treatment displayed an increased paw thickness compared to animals administered Dexamtehasone, providing an immunological benefit.  These data demonstrate a valid methodology for producing an allergic reaction in experimental animals and show that thense animals are sensitive to pharmacological agents that are known to reduce allergic reactions.

 

If you are interested in learning more about Delayed Type Hypersensitivity, please contactmodels@meliordiscovery.com to start the conversation.