Melior Discovery metabolic
Euglycemic/Hyperinsulinemic Clamp Study
The hyperinsulinemic/euglycemic clamp is a standard method for detecting insulin receptor sensitivity and glucose utilization in experimental animals and in the clinic. In this model, insulin is infused intravenously (IV) at a steady rate and blood glucose levels maintained by variable infusion of glucose. At steady-state, the glucose infusion rate (GIR) is equal to tissue glucose uptake. Drugs, such as the thiazolidinones (PPARγ agonists; e.g. rosiglitazone) increase GIR in animals subjected to the euglycemic clamp and this increase in glucose utilization is driven by increased insulin receptor sensitivity.
The data above illustrate how rosiglitazone-treated animals require a significantly higher rate of glucose infusion to maintain steady glucose levels relative to vehicle treated animals. Data are mean ± SEM; ***p<0.001 compared to vehicle.
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