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Melior Discovery motor function

Essential Tremor
Harmaline has been demonstrated to produce a 10-16 Hz tremor in mice, rats, and guinea pigs and is a standard preclinical model of essential tremor (Martin et al, 1986; Handforth, 2012).   Tremor can be assessed via a force plate actimeter and can be blocked or attenuated by propranolol, ethanol, and other alcohols.

In Figure A  below, propranolol (20 mg/kg IP) or saline (Drug 1) were administered following a baseline recording period, and 15’ later, saline or harmaline (15 mg/kg IP) were administered in a crossover design.  Saline or propranalol alone had no effect on tremor, while harmaline alone significantly increased tremor from 10 – 20 minutes after administration.  In contrast, propranolol pre-treatment completely blocked the tremorigenic effect of harmaline.  Similar results were obtained with 10% or 14% ethanol.

 

                             Figure A.  Propranolol blocks harmaline-induced tremor

 

 

                        Harmaline induced tremor

 

Tremor (10-16 Hz) before (baseline; Bsln) and after treatment with saline or propranolol (Drug 1) followed by saline or harmaline (Drug2).   Saline or propranolol alone had no effect on body tremor.   Harmaline alone significantly increased body tremor, while propranolol completely blocked the effect of harmaline.  Values = mean ± SEM.  . **** p < 0.001, 2-way ANOVA.

 

                           

 

If you are interested in learning more about models of tremor, including essential tremor, please contact models@meliordiscovery.com to start the conversation today.