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Melior Discovery neurology

Maximal Electroshock
Epilepsy is a significant medical problem facing millions of people, most of who are not controlled by the current anticonvulsants on the market.  The maximal electroshock seizure (MES) model can effectively test the potential efficacy of a compound for inhibiting tonic seizure.  In this model, the effects of phenytoin at various dose levels of 3, 10 and 30 mg/kg were compared to vehicle treated mice. Animals were treated with either vehicle or Phenytoin and observed for 60 minutes prior to MES.   

                         Maximal Electroshock Phenytoin Dose response

Maximal electroshock Phenytoin dose response. Sixty minutes after vehicle or Phenytoin (3, 10 or 30 mg/kg) treatment, saline was applied to both eyes and electroconvulsions were produced by generating current delivered via corneal electrodes by a Rodent Shocker generator (60 Hz).  A 20 second max-out time was used for the mice that did not seize.  Any seizure activity (i.e. clonic seizures defined as rapid spams or jerky movements of the limbs) prior to tonic hindlimb seizure was recorded as presence of seizure.  Mice were administered an electroshock of 25 mA and the latency to tonic seizure was recorded.    All mice in the vehicle and Phenytoin 3 and 10 mg/kg groups displayed tonic seizure after a few seconds of receiving the current.  Mice that were treated with Phenytoin 30 mg/kg all reached the 20 second time out without displaying signs of a tonic seizure.  Data are mean ± SEM, 20 second time-out; ***p<0.0001 compared to vehicle.

 

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