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melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery neurology

6 Hz Psychomotor Seizure Model

Epilepsy is a significant medical problem facing millions of people, most of who are not controlled by the current anticonvulsants on the market.  Maximal electroshock seizure (MES) is an excellent model that tests a compound’s efficacy on the inhibition of a tonic seizure. In the current model, the effects of Valproate (100 and 300 mg/kg) as an anticonvulsant were tested against vehicle treated mice at differing currents of 22, 32 or 44 mA. The percentage of mice exhibiting seizure was examined.

 

              6Hz seizure model psychomotor seizure

Percentage of mice exhibiting clonus seizure activity.  ICR-CD1 mice (n=8) were treated with either vehicle or Valproate (100 or 300 mg/kg) and administered a 6 Hz corneal stimulation with various currents (44 mA, 32 mA or 22 mA) fifteen minutes after treatment. Mice were monitored for fifteen seconds, post-shock administration, to determine if seizure activity was present.   Seizures were characterized as described in Kaminski et at., 2004 and Barton et al., 2001 and include locomotor agitation, a "stunned" posture associated with rearing (bipedal standing), forelimb automatic movements and clonus, twitching of the vibrissae, and Straub-tail.  Data are mean ± SEM, 15 second time-out; *p<0.05, **p<0.01 and ***p<0.001 compared to vehicle.

If you are interested in learning more about the 6 Hz Seizure Model, please contact models@meliordiscovery.com to start the conversation.