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Melior Discovery neurophysiology

EEG Sleep / Wake and Motor Activity
Cortical activity recorded in rodents can be used to characterize basic behavioral states such as sleep/wake activity, as well as evaluating interactions between drugs and environmental factors such as fatigue.  Rats or mice can be chronically implanted and, depending on treatments, can be tested repeatedly for several months.  General motor activity and body  temperature can also be monitored by implanted transmitters.  The effects of caffeine on wake, sleep (SWS), and rapid-eye movement (REM) sleep activity, and motor activity and body temperature are illustrated below.  See also Cortical EEG Frequency Analysis

Figure A- Effect of Caffeine: Percent Time Awake:

                    

The effect of caffeine (30mg/kg IP) on wake activity in rats is displayed above.  Rats (9 vehicle, 6 caffeine) were evaluated for 24 hours.  The percent time spent awake is shown for each half-hour interval for 10 hours post dosing.  *p<0.05, unpaired t-test. 

 

Figure B- Effect of Caffeine on Wake - SWS - REMS

The mean time spent in wake, slow-wave sleep (SWS), and rapid-eye movement sleep (REMS) for the first 4 hours post dosing is shown in graph above left.  On the right, the time to onset of sleep and REM sleep is shown. *p<0.05, unpaired t-test. 

 

Figure C- Effect of Caffeine on Motor Activity and Temperature


 

Caffeine increased motor activity for 4 hours and body temperature for 2 hours post dosing as shown above.  *p<0.05, unpaired t-test. 

 

If you are interested in learning more about EEG Sleep/Wake and Motor Activity, please contact models@meliordiscovery.com to start the conversation.