Melior Discovery neurophysiology
EEG Pro- and Anti-Convulsant Evaluation
Figure A- EEG Frequency Time Course After PTZ; Diazepam vs Vehicle:
Sub-convulsant dose of PTZ increases low-frequency EEG power at 5 Hz for several hours after administration.  Normal waking EEG pre-dosing. Note peak in FFT record at 7-8 Hz corresponding to theta band.  Diazepam (5mg/kg) alone has little effect on EEG (27 minutes post dosing).  This animal was treated with Vehicle, then 27 minutes later given PTZ (40mg/kg). EEG recorded 26 minutes later shows periodic bursting which produced a significant increase in FFT amplitude at 5 Hz, distinct from theta EEG (note 5x decrease in FFT scale).  Same animal as panel  also received PTZ at the same time as animal in panel . In this case, Diazepam almost completely blocked PTZ-induced burstin.
Figure B- Diazepam Supresses PTZ-Induced EEG Bursting:
Bursting induced by PTZ was quantified by frequency analysis, and the effect of Diazepam evaluated. Graph above shows significant effect of Diazepam (5mg/kg) in suppressing PTZ-induced bursting (paradigm as in Figure A; n=5/group). This paradigm potentially shows repeat testing of animals to determine effects of chronic or sub-chronic administration of PTZ or anti-convulsant drug. *p<0.005.
Figure C- Suppression of PTZ Bursting in Mice by Diazepam:
Bursting can be induced in mice by dose-escalation of PTZ. The graph above shows he increase in EEG power at 4-6 Hz over a period of 280 minutes during successive administration of PTZ doses totaling 140 mg/kg. Diazepam (5 mg/kg) was then administered and within minutes suppressed the bursting.
Figure D- Suppression of PTZ Bursting in Mice by Diazepam:
Quantitative evaluation of bursting in mice and suppresion by Diazepam (5 mg/kg). *p<0.05; ***p<0.001.
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