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Melior Discovery neurophysiology

Subthalamic Nucleus (STN) Unit Recording in 6-OHDA-Lesioned Rats
It has been demonstrated clinically in Parkinson's diesase patients that neurons in the subthalamic nucleus (STN) show an atypical bursting pattern.  Reduction of STN bursting by lesions or deep-brain stimulation has been demonstrated to ameliorate Parkinsonian symptoms in humans and monkeys (Patel et al. 2003a, Patel et al., 2003b).  STN burst firing has also been demonstrated in animal models in which nigro-striatal dopaminergic pathways have been chemically ablated, such as the rat 6-OHDA lesion model. This model may thus be valuable for evaluating therapeutic treatments for Parkinson's disease.

Figure A- Single Unit Recording in a Normal Rat:

Single Unit Recording in a Normal Rat

Acute recording from an STN neuron using stereotaxically implanted metal electrode in an anesthetized normal rat.  Note steady firing rate.

 

Figure B- Single Unit Recording in 6-OHDA-Lesioned Rat:

   Single Unit Recording in 6-OHDA-Lesioned Rat

Isolated spike from an STN neuron in a 6-OHDA-lesioned rat.  Spike has characteristic biphasic shape with slight "tail."

 

Figure 3- Single Unit Recording in a 6-OHDA-Lesioned Rat:

Single Unit Recording in 6-OHDA-Lesioned Rat                                                         

Clear bursting activity was observed in STN cells in this rat in which the dopaminergic pathway was lesioned by stereotaxic injection of 6-OHDA into the medial-forebrain bundle (MFB).  The lesion was comfirmed by apomorphine-induced rotations four weeks later.  Bursting parameters are quantified off-line to determine drug treatment effects

 

Figure 4: Localization of Recording Site in the STN

Histology- Localization of Recording Site in the STN

At the end of the recording session, pontamine sky-blue was iontophoretically injected into the recording site (white arrow) to confirm recording from the STN. 

 

If you are interested in learning more about Subthalamic Nucleus Single Unit Recording, please contact models@meliordiscovery.com to start the conversation today.