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Melior Discovery pain/neuropathic

von Frey / Carrageenan sensitivity: an assesment of allodynia
Allodynia is an abnormal nociceptive response to a stimulus that does not provoke pain under normal conditions.  This model is included in our battery of analgesia assays as a touch sensitivity assay using Semmes-Weinstein monofilaments (i.e. von Frey filaments).  This assay assesses the sensitivity and pain responses of Carrageenan-challenged mice to thin hair-like filaments of varying diameter and corresponding force needed to bend the fiber. 

Intraplantar Carrageenan has been demonstrated to increase sensitivity of the mouse hindpaw and is commonly used in allodynia assays.   Von Frey fialments are typically used to ascertain the mechanoreceptor threshold level of a mouse and the ability of a test agent to affect the mechanoreceptor response.  A number of pharmacological agents are effective in reducing sensitivity of mice to von Frey stimuli including serotonin reuptake inhibitors, anti-inflammatory agents, GABA enhancers and opiods. This assay is included in the battery of analgesia assays that include the thermal nociceptive assay and the formalin chemical nociceptive assay

 

                             von Frey Carrageenan allodynia assay

One hour prior to testing, animals were injected with 1% carrageenan into the right hindpaw and acclimated to the testing environment.  Thirty minutes prior to testing, mice were injected with vehicle or Indomethacin.  Two hours post-Carrageenan challenge, von Frey mechanical fibers are applied to the plantar surface of each paw to ellicit a nociceptive response.  Indomethacin significantly increased mechanical thresholds post-carageenan challenge compared to vehicle treated mice. Data are mean ± SEM; ***p<0.0001 compared to vehicle.

 

                       von Frey Carrageenan allodynia assay baseline comparison

An additional comparison was performed to evaluate differences at baseline vs. post-Carrageenan challenge. Both treatment groups prior to dosing had similar thresholds at baseline. However, two hours after the Carrageenan challenge, vehicle treated mice had significantly reduced thresholds compared to Indomethacin treated mice. Data are mean ± SEM; *p<0.05 compared to baseline vehicle.

 

If you are interested in learning more about von Frey/ Carrageenan sensitivity assay, please contact models@meliordiscovery.com to start the conversation today.