melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling
Melior Discovery
home
Therapeutic Areas
Safety Assessment
 

theraTRACE

Melior Pharmaceuticals

              

 

             

 

                 

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery pain/neuropathic

Hot Plate Analgesia Assay
The thermal analgesia hot plate assay is a pharmacological test for evaluating the analgesic potential of test compounds. A number of clinically approved pharmacological agents have been demonstrated to delay the onset of sensitivity upon paw exposure to heat including opioids and GABA potentiators.  Typically, the hot plate assay is included in a battery of assays evaluating the analgesic potential of test compounds. In this study, the thermal analgesia test is reported with the formalin chemical nociception assay and the von Frey touch sensitivity test

                               Hot plate analgesia assay latency to pain behavior

Latency to pain behavior.  30 minutes after treatment with either vehicle or Morphine, mice were placed on a 52°C hot plate apparatus to measure the latency of first hind paw pain response.  This response was ellicted by a paw lick, shake or escape jump.  Mice treated with Morphine had significantly increased latencies to respond to thermal stimulation compared to mice treated with vehicle. 

 

The mouse hot plate analgesia assay is a test of painful stimulation from heat sensitivity.  Animals that received vehicle displayed an average pain response of 15 seconds.  When administered Morphine, the pain response was significantly delayed, providing an analgesic effect.  Data are mean ± SEM; **p<0.01 compared to vehicle.

 

If you are are interested in learning more about the Hot Plate Analgesia Assay, please contact models@meliordiscovery.com to start the conversation.