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Melior Pharmaceuticals

melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery drug abuse liability

Conditioned Place Preference

The Conditioned Place Preference (CPP) model is a standard preclinical behavioral model used to measure the rewarding effects of drugs.  This model allows measurement of the reinforcing properties of a drug in the absence of the drug itself.  Animals learn, through Pavlovian conditioning, to associate a particular location with a particular stimulus in contrast to a location where the stimulus is absent. 

 

On a test day, the animals are allowed to choose the side they prefer, and in the case of drugs with known abuse potential, this is usually the side associated with the drug during the training sessions.  In this study, hydrocodone was used to determine its ability to induce conditioned place preference.  This study measured the time spent in the drug-paired chamber in pre- and post- conditioning sessions.

 

Figure 1: Conditioned Place Preference with Hydrocodone

 

                          Conditioned Place Preference with Hydrocodone - Abuse Liability Study

Conditioned Place Preference with Hydrocodone.  Hydrocodone 5mpk dose causes a significant increase in time spent in the drug-paired compartment on the post conditioning day when compared to the pre-conditioning day.  Data is mean ± SEM; *p<0.05 compared to pre-conditioning; n=8.

 

Figure 2: Hydrocodone Dose Response Curve

                             Conditioned Place Preference Hydrocodone Dose Response Curve - Abuse Liability

Dose Response Curve.  Hydrocodone caused a significant increase in time spent in the drug-paired compartment on post-conditioning day compared to vehicle.  Hydrocodone 1mpk and 3mpk treated animals resulted in a signficiantly higher time in drug paired side while 5mpk treated animals resulted in an increased trend in time spent in drug paired side. This result indicates that hydrocodone induces a conditioned place preference in rats.  Data is mean ± SEM; *p<0.05 compared to pre-conditioning; n=2.

 

If you are interested in learning more about Conditioned Place Preference Abuse Liability Studies, please contact models@meliordiscovery.com to start the conversation.