6-Hz Psychomotor Seizure
Discover how Melior’s unique phenotypic screening platforms can uncover the untapped value of your candidate therapeutic
Epilepsy is a significant medical problem facing millions of people, most of who are not controlled by the current anticonvulsants on the market. Although a number of marketed anticonvulsant agents exist, there remains a high unmet medical need for better agents with reduced liability.
The 6 Hz Seizure Model is categorized as a partial or focal form of seizure (Psychomotor Seizure). This Psychomotor Seizure procedure is a model that tests a compound’s efficacy on the inhibition of certain forms of seizure. When characterizing anti-seizure candidates it an excellent screening complement to models of generalized seizure such as PTZ or MES.
In the current model, the effects of Valproate (100 and 300 mg/kg) as an anticonvulsant were tested against vehicle treated mice at differing currents of 22, 32 or 44 mA. The percentage of mice exhibiting seizure was examined.
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Percentage of mice exhibiting clonus seizure activity. ICR-CD1 mice (n=8) were treated with either vehicle or Valproate (100 or 300 mg/kg) and administered a 6 Hz corneal stimulation with various currents (44 mA, 32 mA or 22 mA) fifteen minutes after treatment. Mice were monitored for fifteen seconds, post-shock administration, to determine if seizure activity was present.
Seizures were characterized as described in Kaminski et al., 2004 and Barton et al., 2001 and include locomotor agitation, a “stunned” posture associated with rearing (bipedal standing), forelimb automatic movements and clonus, twitching of the vibrissae, and Straub-tail. Data are mean ± SEM, 15 second time-out; *p<0.05, **p<0.01 and ***p<0.001 compared to vehicle.
Interested in running a 6-Hz Psychomotor Seizure study?
Interested in running a 6-Hz Psychomotor Seizure study?If you are interested in learning more about the 6-Hz Psychomotor Seizure model, please contact us to start the conversation today!