Melior Discovery inflammation/arthritis
Zymosan-A Induced Peritonitis
Leukocyte infiltration into a site of injury or irritation is an integral part of an inflammatory process associated with the peritonitis response. The peritonitis model described in this report utilizes Zymosan-A to induce leukocyte accumulation in the peritoneum. This model can be can be utilized as a model of inflammation, acute peritonitis and polymorphonucleocyte migration. Importantly, this model is an effective model for detecting a test compounds ability to attenuate inflammatory processes associated with peritonitis. Anti-inflammatory agents including steroidal and non-steroidal anti-inflammatory compounds are effective in reducing leukocyte recruitment and/or infiltration in this model.
Prednisone Administration Reduces Zymosan-A Mediated increases in Leukocyte Number
Cell-type |
Untreated |
Vehicle |
Prednisone (20 mg/kg) |
Leukocytes |
2.98 ± 0.94 |
9.22 ± 1.45* |
4.90 ± 1.01a |
Neutrophils |
0.74 ± 0.38 |
3.42 ± 0.69* |
1.51 ± 0.36a |
Lymphocytes |
1.17± 0.52 |
2.91 ± 0.38* |
1.90 ± 0.39 |
Monocytes |
0.36 ± 0.17 |
2.17 ± 0.41* |
1.08 ± 0.21a |
Eosinophils |
0.17 ± 0.10 |
0.61 ± 0.15* |
0.38 ± 0.14 |
Basophils |
0.06 ± 0.03 |
0.13 ± 0.03* |
0.04 ± 0.06a |
PMN’s |
0.97 ± 0.51 |
4.15 ± 0.85* |
1.93 ± 0.50a |
If you are interested in learning more about the Zymosan-A Induced Peritonitis assay, please contact models@meliordiscovery.com to start the conversation.
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