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 melior melior melior discovery melior pharmaceuticals preclinical services pre-clinical services in vivo pharmacology in vivo efficacy efficacy models pharmacokinetics indications discovery specialized animal models bioanalytical services theratrace exton pennsylvania cro contract research drug discovery drug development metabolic disease alzheimer’s alzheimers diabetes reprofiling

Melior Discovery inflammation/arthritis

Zymosan-A Induced Peritonitis
Leukocyte infiltration into a site of injury or irritation is an integral part of an inflammatory process associated with the peritonitis response. The peritonitis model described in this report utilizes Zymosan-A to induce leukocyte accumulation in the peritoneum. This model can be can be utilized as a model of inflammation, acute peritonitis and polymorphonucleocyte migration. Importantly, this model is an effective model for detecting a test compounds ability to attenuate inflammatory processes associated with peritonitis. Anti-inflammatory agents including steroidal and non-steroidal anti-inflammatory compounds are effective in reducing leukocyte recruitment and/or infiltration in this model.

Prednisone Administration Reduces Zymosan-A Mediated increases in Leukocyte Number

Cell-type Untreated Vehicle Prednisone (20 mg/kg)
Leukocytes 2.98 ± 0.94 9.22 ± 1.45* 4.90 ± 1.01a
Neutrophils 0.74 ± 0.38 3.42 ± 0.69* 1.51 ± 0.36a
Lymphocytes 1.17± 0.52 2.91 ± 0.38* 1.90 ± 0.39
Monocytes 0.36 ± 0.17 2.17 ± 0.41* 1.08 ± 0.21a
Eosinophils 0.17 ± 0.10 0.61 ± 0.15* 0.38 ± 0.14
Basophils 0.06 ± 0.03 0.13 ± 0.03* 0.04 ± 0.06a
PMN’s 0.97 ± 0.51 4.15 ± 0.85* 1.93 ± 0.50a

If you are interested in learning more about the Zymosan-A Induced Peritonitis assay, please contact models@meliordiscovery.com to start the conversation.